HA tag Rabbit Monoclonal Antibody(ARB574)
Datasheet 
Key features and details
- Target:
- Clone ID:
- Source/Host:
- Reactivity:
- Applications:
- Clonality:
- Storage:
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Brand:
Product Details
Application | Dilution Ratio |
IHC | 1:100 - 1:200 |
IF/ICC | 1:400 - 1:2000 |
FC | 1:400 - 1:2000 |
IP | 1:5 - 1:10 |
Predicted Molecular Wt | Depending on customers’ target of interest |
Species Cross-reactivity | Species independent |
Purity | ProA affinity purified IgG |
Swissprot ID | N/A |
Immunogen | YPYDVPDYA (Influenza hemagglutinin-HA-epitope) conjugated to KLH |
Storage Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Predicted MW: Depend on fusion protein with HA Tag
Lane 1: 293 cells lysate transfected with C-terminal HA tagged gene (at 1:2,000 dilution).
Lane 2: 293 cells lysate transfected with N-terminal HA tagged gene (at 1:1,000 dilution).
Lane 3: 293 cells lysate without any transfection (at 1:400 dilution).
Lane 1: 1 μg per lane
Lane 2/3: 10 μg per lane
2nd Ab:
GAR HRP(H+L) 1:5,000
Overlay histogram showing 293 cells transfected with N-terminal (Green) and C-terminal (Red) HA tagged gene stained with ARB574. The cells were then incubated in the antibody (ARB574, 1:2,000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
ARB574 staining HA tag in 293 cells transfected with N-terminal HA tagged gene by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:2,000) at 4°C. An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG (1:500).
ARB574 staining HA tag in 293 cells transfected with C-terminal HA tagged gene by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:2,000) at 4°C. An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG.
HA tag was immunoprecipitated from 0.2mg of 293 whole cells lysate transfected with N-terminal HA tagged gene with ARB574 at 1:10 dilution. 2nd Ab: GAR HRP for IP 1:500 Lane 1: ARB574 IP in 293 whole cell lysate transfected with N-terminal HA tagged gene Lane 2: PBS instead of ARB574 in 293 whole cell lysate transfected with N-terminal HA tagged gene Lane 3: 293 whole cell lysate transfected with N-terminal HA tagged gene, 10 μg (input) Exposure: 60s
HA tag was immunoprecipitated from 0.2mg of 293 whole cells lysate transfected with C-terminal HA tagged gene with ARB574 at 1:10 dilution. 2nd Ab: GAR HRP for IP 1:500 Lane 1: ARB574 IP in 293 whole cell lysate transfected with C-terminal HA tagged gene Lane 2: PBS instead of ARB574 in 293 whole cell lysate transfected with C-terminal HA tagged gene Lane 3: 293 whole cell lysate transfected with C-terminal HA tagged gene, 10 μg (input) Exposure: 60s
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