HA tag Rabbit Monoclonal Antibody(ARA782)

Datasheet

MSDS

HA tag Rabbit Monoclonal Antibody(ARA782)

Key features and details

Target: HA tag
Source/Host: Rabbit
Reactivity: Species independent
Clonality: Monoclonal
Applications: WB,IF/ICC,FC,IP
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : ARA6569

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

HA tag Recombinant Rabbit Monoclonal Antibody Product Datasheet Predicted Molecular Wt: Depending on sample to be analyzedkDa Purity: ProA affinity purified IgG Species independent Species Cross-reactivity: Form: Liquid Species cross-reactivity determined by WB Swissprot ID: N/A Applications: WB IF/ICC FC IP Background: Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag derived from the HA molecule corresponding to amino acids

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:1000-1:2000
IF/ICC	1:400-1:2000
FC	1:400-1:2000
IP	1:10

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit Monoclonal Antibody to HA tag
Antibody Type	Primary antibody
Predicted MW
Immunogen	YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH.
Purification	ProA affinity purified IgG
Form/Buffer	PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.57%.
Alternative Names	HA epitope tag; hemagglutinin

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Predicted MW: Depend on fusion protein with HA tag
Lane 1: 293 cells lysate transfected with C-terminal HA tagged gene (ARA782 at 1:2,000 dilution).
Lane 2: 293 cells lysate transfected with N-terminal HA tagged gene (ARA782 at 1:1,000 dilution).
Lane 3: 293 cells lysate without any transfection (ARA782 at 1:400 dilution).
Lane 1: 1 µg per lane
Lane 2/3: 10 µg per lane
2nd Ab:
GAR HRP(H+L) 1:5,000
Exposure: 20s

Overlay histogram showing 293 cells transfected with N-terminal (Green) and C-terminal (Red) HA tagged gene stained with ARA782. The cells were then incubated in the antibody (ARA782, 1:2,000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.

ARA782 staining HA tag in 293 cells transfected with N-terminal HA tagged gene by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:2,000) at 4°C. An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain.

Control: PBS and secondary antibody, An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG (1:500).

ARA782 staining HA tag in 293 cells transfected with C-terminal HA tagged gene by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:2,000) at 4°C. An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain.

Control: PBS and secondary antibody, An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG (1:500).

HA tag was immunoprecipitated from 0.2mg of 293 whole cells lysate transfected with N-terminal HA tagged gene with ARA782 at 1:10 dilution.
2nd Ab:
GAR HRP for IP 1:500

Lane 1: ARA782 IP in 293 whole cell lysate transfected with N-terminal HA tagged gene
Lane 2: PBS instead of ARA782 in 293 whole cell lysate transfected with N-terminal HA tagged gene
Lane 3: 293 whole cell lysate transfected with N-terminal HA tagged gene, 10 µg (input)

Exposure: 60s

HA tag was immunoprecipitated from 0.2mg of 293 whole cells lysate transfected with C-terminal HA tagged gene with ARA782 at 1:10 dilution.
2nd Ab:
GAR HRP for IP 1:500

Lane 1: ARA782 IP in 293 whole cell lysate transfected with C-terminal HA tagged gene
Lane 2: PBS instead of ARA782 in 293 whole cell lysate transfected with C-terminal HA tagged gene
Lane 3: 293 whole cell lysate transfected with C-terminal HA tagged gene, 10 µg (input)

Exposure: 60s

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

New Products

TRBC1 Mouse Monoclonal Antibody(ARA662)

Mouse monoclonal to TRBC1

OCT2 Mouse Monoclonal Antibody(C2325)

Mouse monoclonal antibody to OCT2

ARExVisual® DAB Buffer

Primary Antibody Diluent

ARExVisual® DAB chromogen (20×)

CD68 Rabbit Monoclonal Antibody(ARA633)