GRP3 Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:2000 |
IF/ICC | 1:50 - 1:200 |
Description | Rabbit polyclonal antibody to GRP3 |
Specificity | Recognizes endogenous levels of GRP3 protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human GRP3 |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 78 kD; Observed: 78 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | GRP3; KIAA0846; Ras guanyl-releasing protein 3; Calcium and DAG-regulated guanine nucleotide exchange factor III; Guanine nucleotide exchange factor for Rap1 |
Gene Symbol | RASGRP3 |
Entrez Gene | 25780(Human) |
SwissProt | Q8IV61(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of GRP3 expression in BT474 (A), A549 (B), mouse heart (C), mouse lung (D) whole cell lysates. (Predicted band size: 78 kD; Observed band size: 78 kD)
Immunofluorescent analysis of GRP3 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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