GATA2/3 Rabbit Monoclonal Antibody(C1081)
Datasheet 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:200 |
Description | Recombinant rabbit monoclonal antibody to GATA2/3 |
Specificity | Recognizes endogenous levels of GATA2/3 protein |
Antibody Type | Primary antibody, Recombinant |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within human GATA2/3. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 50; Observed: 51 kD |
Form/Buffer | Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. |
Alternative Names | GATA2; Endothelial transcription factor GATA-2; GATA-binding protein 2; GATA3; Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3 |
Gene Symbol | GATA2; GATA3 |
Entrez Gene | 2624; 2625(Human) |
SwissProt | P23769; P23771(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of GATA2/3 expression in HEK293T (A), THP1 (B), K562 (C) whole cell lysates. (Predicted band size: 50; 47 kD; Observed band size: 51 kD)
Immunofluorescent analysis of GATA2/3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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