GAP43 (Phospho-S41) Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
-
Brand:
Product Details
WB | 1:500 - 1:2000 |
IF/ICC | 1:50 - 1:100 |
Description | Rabbit polyclonal antibody to GAP43 (Phospho-S41) |
Specificity | Recognizes endogenous levels of GAP43 protein only when phosphorylated at S41. |
Antibody Type | Primary antibody |
Imnunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S41 of human GAP43 protein. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 24 kD; Observed: 24 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | Neuromodulin; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50; pp46 |
Gene Symbol | GAP43 |
Entrez Gene | 2596(Human); 14432(Mouse); 29423(Rat) |
SwissProt | P17677(Human); P06837(Mouse); P07936(Rat) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of GAP43 (Phospho-S41) expression in HeLa PMA-treated (A), SHSY5Y (B) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 24 kD)
Immunofluorescent analysis of GAP43 (Phospho-S41) staining in SHSY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
FAQs
New Products
