FOXO3 (Phospho-S253) Rabbit Monoclonal Antibody(C1498)

MSDS

FOXO3 (Phospho-S253) Rabbit Monoclonal Antibody(C1498)

Key features and details

Recombinant rabbit monoclonal antibody to FOXO3 (Phospho-S253)

Target: FOXO3 (Phospho-S253)
Source/Host: Rabbit
Reactivity: Human, Mouse, Rat
Clonality: Monoclonal
Applications: WB
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : AMA01110

US$ Please choose

Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Transcriptional activator that recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3' and regulates different processes, such as apoptosis and autophagy . Acts as a positive regulator of autophagy in skeletal muscle: in starved cells, enters the nucleus following dephosphorylation and binds the promoters of autophagy genes, such as GABARAP1L, MAP1LC3B and ATG12, thereby activating their expression, resulting in proteolysis of skeletal muscle proteins . Triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress .

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Recombinant rabbit monoclonal antibody to FOXO3 (Phospho-S253)
Specificity	Recognizes endogenous levels of FOXO3 protein only when phosphorylated at S253
Antibody Type	Primary antibody, Recombinant
Imnunogen	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S253 of human FOXO3 protein. The exact sequence is proprietary.
Purification	The antibody was purified by immunogen affinity chromatography.
Molecular Weight	Predicted: 71 kD; Observed: 97 kD
Form/Buffer	Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Alternative Names	FKHRL1; FOXO3A; Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1
Gene Symbol	FOXO3
Entrez Gene	2309(Human); 56484(Mouse)
SwissProt	O43524(Human); Q9WVH4(Mouse)

*AREX continuously optimizes its products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor. Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of FOXO3 (Phospho-S253) expression in HEK293T (A), SHSY5Y (B), A431 (C), mouse liver (D), mouse kidney (E), rat liver (F) whole cell lysates. (Predicted band size: 71 kD; Observed band size: 97 kD)

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for use in diagnostic procedures.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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