FIBP Rabbit Polyclonal Antibody

Key features and details

Rabbit polyclonal antibody to FIBP
  • Target: FIBP
  • Source/Host: Rabbit
  • Reactivity: Human
  • Clonality: Polyclonal
  • Applications: WB
  • Conjugation: Unconjugated
  • Storage: at-20°C
  • Brand:
CAT.NO. : APA12898
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Product Details
Background
May be involved in mitogenic function of FGF1. May mediate with IER2 FGF-signaling in the establishment of laterality in the embryo .
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB

1:500 - 1:1000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Overview

Description

Rabbit polyclonal antibody to FIBP

Specificity

Recognizes endogenous levels of FIBP protein.

Antibody Type

Primary antibody

Imnunogen

Recombinant fusion protein of human FIBP

Purification

The antibody was purified by immunogen affinity chromatography.

Molecular Weight

Predicted: 41 kD; Observed: 42 kD

Form/Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Alternative Names

Acidic fibroblast growth factor intracellular-binding protein; aFGF intracellular-binding protein; FGF-1 intracellular-binding protein

Gene Symbol

FIBP

Entrez Gene

9158(Human); 58249(Mouse)

SwissProt

O43427(Human); Q9JI19(Mouse)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data

Western blot analysis of FIBP expression in SW620 (A), MCF7 (B), rat brain (C) whole cell lysates. (Predicted band size: 41 kD; Observed band size: 42 kD)

Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for use in diagnostic procedures.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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