Exosome CD81 Protein Detection Kit
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CAT.NO. : UR52343
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Product Details
Background
The Exosome CD81 Protein Detection Kit is a convenient and efficient product developed by Shanghai Umibio Biotechnology Co., Ltd. for the detection of CD81, a specific marker protein of exosomes. The exosome‑specific lysis buffer included in the kit efficiently lyses exosomes to release their proteins. The CD81 positive control, along with the primary and secondary antibodies, allows monitoring of the experimental system and accurate detection of CD81 protein in exosome samples.
Product Composition
| Component Name | UR52341 | UR52343 |
|---|---|---|
| Exosome Protein Lysis Buffer | 5 mL | 20 mL |
| CD81 Positive Control | 100 μL | 100 μL × 4 tubes |
| CD81 antibody | 10 μL | 40 μL |
| Goat anti-Rabbit IgG antibody (HRP) | 10 μL | 40 μL |
Product Advantages
High Specificity & Sensitivity: The CD81 nanobody is a recombinant alpaca VHH fused with a rabbit IgG Fc domain. It detects stronger specific signals in exosome lysates and exhibits no cross-reactivity with other proteins.
Versatile Application: Suitable for both WB (Western Blot) and ELISA detection.
Cost-Effective: Available at a competitive price.
Versatile Application: Suitable for both WB (Western Blot) and ELISA detection.
Cost-Effective: Available at a competitive price.
Data
Annotation: PC refers to the CD81 positive control.
Experimental Conditions:
SDS-PAGE gel: 10% separation gel
Sample loading: 10 μL of PC; 10 μg of 293T Cell Exosomes
Primary antibody: CD81 antibody, diluted at 1:1000, incubated overnight at 4°C
Secondary antibody: Goat anti-Rabbit IgG, diluted at 1:2000, incubated at room temperature for 50 min
Storage
-20℃
Shipping temp:
Ship on Ice
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