ETS1 Mouse Monoclonal Antibody(C3687)

Key features and details

Mouse monoclonal antibody to ETS1
  • Target: ETS1
  • Source/Host: Mouse
  • Reactivity: Human
  • Clonality: Monoclonal
  • Applications: WB, FC
  • Conjugation: Unconjugated
  • Storage: at-20°C
  • Brand:
CAT.NO. : AMA03299
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Product Details
Background
Transcription factor . Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts . May control the differentiation, survival and proliferation of lymphoid cells . May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion .
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB

1:500 - 1:1000

IF/ICC

1:10 - 1:50

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Overview

Description

Mouse monoclonal antibody to ETS1

Specificity

Recognizes endogenous levels of ETS1 protein.

Antibody Type

Primary antibody

Imnunogen

Recombinant fusion protein of human ETS1. The exact sequence is proprietary.

Purification

This antibody is purified through a protein G column.

Molecular Weight

Predicted: 50 kD; Observed: 55 kD

Form/Buffer

Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Alternative Names

EWSR2; Protein C-ets-1; p54

Gene Symbol

ETS1

Entrez Gene

2113(Human)

SwissProt

P14921(Human)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data

Western blot analysis of ETS1 expression in MOLT4 (A) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 55 kD)

Flow cytometric analysis of K562 cells using Anti-ETS1 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AREX® Fluor 488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for use in diagnostic procedures.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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