ERK1 Mouse Monoclonal Antibody(C3747)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IHC | 1:50 - 1:200 |
IF/ICC | 1:10 - 1:50 |
Description | Mouse monoclonal antibody to ERK1 |
Specificity | Recognizes endogenous levels of ERK1 protein. |
Antibody Type | Primary antibody |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ERK1. The exact sequence is proprietary. |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 43 kD; Observed: 44 kD |
Form/Buffer | Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | ERK1; PRKM3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p44-ERK1 |
Gene Symbol | MAPK3 |
Entrez Gene | 5595(Human); 26417(Mouse) |
SwissProt | P27361(Human); Q63844(Mouse) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of ERK1 expression in MCF7 (A), Hela (B), Jurkat (C), mouse brain (D) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 44 kD)
Immunohistochemical analysis of ERK1 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of ERK1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Western blot analysis of ERK1 expression in wild type (WT) and knockdown (KD) HeLa cell lysates.
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