EMR2 Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:100 - 1:500 |
Description | Rabbit polyclonal antibody to EMR2 |
Specificity | Recognizes endogenous levels of EMR2 protein. |
Antibody Type | Primary antibody |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EMR2. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 90 kD; Observed: 90; 38 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | EMR2; EGF-like module-containing mucin-like hormone receptor-like 2; EGF-like module receptor 2; CD312; Adhesion G protein-coupled receptor E2 |
Gene Symbol | ADGRE2 |
Entrez Gene | 30817(Human) |
SwissProt | Q9UHX3(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of EMR2 expression in DLD (A), mouse spleen (B), rat spleen (C) whole cell lysates. (Predicted band size: 90 kD; Observed band size: 90; 38 kD)
Immunofluorescent analysis of EMR2 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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