Desmoplakin Rabbit Polyclonal Antibody
MSDS 
Key features and details
Rabbit polyclonal antibody to Desmoplakin
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
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Brand:
CAT.NO. : APA13124
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Product Details
Background
A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion . Critical for cell-cell adhesion in early stage blastocysts and progression through proamniotic cavity formation . Not required for preimplantation morphogenic process in blastocysts . Required for keratin filament anchoring at the desmosome junction and subsequent organization of the keratin intermediate filament network within the cytoplasm . Required for anchoring of desmosomes to the microtubule architecture, via its interaction with NIN . Plays a key role in adhesion and organization of the dermal epithelial barrier . Critical for the maintenance of the neural tube structure following formation and organization of the neuroepithelium .
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:1000 |
Overview
Description | Rabbit polyclonal antibody to Desmoplakin |
Specificity | Recognizes endogenous levels of Desmoplakin protein. |
Antibody Type | Primary antibody |
Imnunogen | KLH-conjugated synthetic peptide of human Desmoplakin |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 260; Observed: 330; 280 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | Desmoplakin; DP; 250/210 kDa paraneoplastic pemphigus antigen |
Gene Symbol | DSP |
Entrez Gene | 1832(Human); 109620(Mouse) |
SwissProt | P15924(Human); E9Q557(Mouse) |
Data
Western blot analysis of Desmoplakin expression in MCF7 (A), Hela (B), mouse heart (C) whole cell lysates. (Predicted band size: 260; 278; 331 kD; Observed band size: 330; 280 kD)
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for use in diagnostic procedures.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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