DDX3X Mouse Monoclonal Antibody(C3393)

MSDS

Key features and details

Mouse monoclonal antibody to DDX3X

Target: DDX3X
Source/Host: Mouse
Reactivity: Human, Mouse, Rat, Monkey
Clonality: Monoclonal
Applications: WB, IF/ICC, IP, ChIP
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : AMA03005

US$ Please choose

Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Multifunctional ATP-dependent RNA helicase . The ATPase activity can be stimulated by various ribo-and deoxynucleic acids indicative for a relaxed substrate specificity . In vitro can unwind partially double-stranded DNA with a preference for 5'-single-stranded DNA overhangs . Binds RNA G-quadruplex (rG4s) structures, including those located in the 5'-UTR of NRAS mRNA . Involved in many cellular processes, which do not necessarily require its ATPase/helicase catalytic activities (Probable). Involved in transcription regulation . Positively regulates CDKN1A/WAF1/CIP1 transcription in an SP1-dependent manner, hence inhibits cell growth. This function requires its ATPase, but not helicase activity . CDKN1A up-regulation may be cell-type specific .

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IF/ICC	1:50 - 1:100
IP	1:10 - 1:50
ChIP	1:10 - 1:50

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Mouse monoclonal antibody to DDX3X
Specificity	Recognizes endogenous levels of DDX3X protein.
Antibody Type	Primary antibody
Imnunogen	Purified recombinant human DDX3 protein fragments expressed in E.coli.
Purification	The antibody was purified by immunogen affinity chromatography.
Molecular Weight	Predicted: 73 kD; Observed: 73 kD
Form/Buffer	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Alternative Names	DBX; DDX3; ATP-dependent RNA helicase DDX3X; DEAD box protein 3 X-chromosomal; DEAD box X isoform; Helicase-like protein 2; HLP2
Gene Symbol	DDX3X
Entrez Gene	1654(Human); 13205(Mouse)
SwissProt	O00571(Human); Q62167(Mouse)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of DDX3X expression in Hela (A), NIH3T3 (B), C6 (C), COS7 (D), K562 (E), Jurkat (F) whole cell lysates. (Predicted band size: 73 kD; Observed band size: 73 kD)

Immunofluorescent analysis of DDX3X staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark.

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for use in diagnostic procedures.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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