Cytokeratin 6 Mouse Monoclonal Antibody(C2810)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
-
Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:100 - 1:500 |
FC | 1:100 - 1:200 |
Description | Mouse monoclonal to Cytokeratin 6 |
Specificity | Recognizes endogenous levels of Cytokeratin 6 protein |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human Cytokeratin 6 expressed in E. Coli |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 60 kD; Observed: 58 kD kD |
Form/Buffer | Mouse IgG2b. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | K6A; KRT6D; Keratin, type II cytoskeletal 6A; Cytokeratin-6A; CK-6A; Cytokeratin-6D; CK-6D; Keratin-6A; K6A; Type-II keratin Kb6; allergen Hom s 5 |
Gene Symbol | KRT6A |
Entrez Gene | 3853(Human) |
SwissProt | P02538(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of Cytokeratin 6 expression in Hela (A), MCF7 (B), SW480 (C), HepG2 (D), T47D (E) whole cell lysates. (Predicted band size: 60 kD; Observed band size: 58 kD kD)
Immunofluorescent analysis of Cytokeratin 6 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of HeLa cells using Anti-Cytokeratin 6 Antibody (green) and negative control (red).
FAQs
New Products
