CLGN Mouse Monoclonal Antibody(C2645)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IHC | 1:100 - 1:500 |
IF/ICC | 1:50 - 1:100 |
FC | 1:100 - 1:200 |
Description | Mouse monoclonal to CLGN |
Specificity | Recognizes endogenous levels of CLGN protein |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human CLGN expressed in E. Coli |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 70 kD; Observed: 72 kD kD |
Form/Buffer | Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | Calmegin |
Gene Symbol | CLGN |
Entrez Gene | 1047(Human) |
SwissProt | O14967(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of CLGN expression in LNCaP (A), HepG2 (B), PC3 (C), Rai (D) whole cell lysates. (Predicted band size: 70 kD; Observed band size: 72 kD kD)
Immunohistochemical analysis of CLGN staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of CLGN staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of HEK293 cells using Anti-CLGN Antibody (green) and negative control (red).
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