CDX2 Rabbit Monoclonal Antibody(ARB826)
Datasheet 
Key features and details
- Target:
- Clone ID:
- Source/Host:
- Reactivity:
- Applications:
- Dilution:
- Clonality:
- Storage:
-
Brand:
CAT.NO. : ARB6616
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Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
CDX2 is a homeobox gene encoding nuclear homeodomain transcriptional factor essential to intestinal organogenesis. CDX2 is crucial for differentiation of epithelium in the intestine, pancreas and biliary tract. CDX2 interacts with the tumour suppressor genes APC and E-cadherin, as well as bcl-2.In the intestine (from duodenum to rectum) CDX2 nuclear protein is demonstrated strongly and uniformly in all types of epithelial cells. In pancreas and biliary tract CDX2 protein is demonstrated in scattered ductal cells. In tumor tissues, CDX2 is positive in the large majority cases of colorectal and appendiceal adenocarcinomas.CDX2 is a relatively sensitive and specific marker for “intestinal” adenocarcinomas. In the classification of (adeno-)carcinomas of the unknown primary, CDX2 is an important marker but should always be included in a panel. Also in order to identify the origin of neuroendocrine tumors, CDX2 is useful in a panel.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
IHC | 1:100 - 1:200 |
Overview
Predicted Molecular Wt | 34 kDa |
Purity | ProA affinity purified IgG |
Subcellular location | Nucleus |
Swissprot ID | Q99626 |
Immunogen | Synthetic peptide corresponding to CDX2 residues within aa1-100 of CDX2 |
Storage Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
Recommended method | Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18° C-25° C) for 30 minutes. |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Immunohistochemical staining of human colonic tissue sections was performed using CDX2 Rabbit Monoclonal Antibody (ARB826)Immunohistochemical staining of human colorectal carcinoma tissue sections was performed using CDX2 Rabbit Monoclonal Antibody (ARB826).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?
AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.
What development scenarios are pathology raw antibodies mainly used for?
Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.
How can I evaluate whether a raw antibody is suitable for our staining platform?
It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.
Can you provide samples for platform validation?
Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.
How should raw antibodies be paired with detection systems?
AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.
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