CDK5 Mouse Monoclonal Antibody(C3318)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:100 |
Description | Mouse monoclonal antibody to CDK5 |
Specificity | Recognizes endogenous levels of CDK5 protein. |
Antibody Type | Primary antibody |
Imnunogen | Purified recombinant human CDK5(N-terminus) protein fragments expressed in E.coli. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 33 kD; Observed: 36 kD |
Form/Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
Alternative Names | CDKN5; Cyclin-dependent kinase 5; Cell division protein kinase 5; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunit |
Gene Symbol | CDK5 |
Entrez Gene | 1020(Human); 12568(Mouse); 140908(Rat) |
SwissProt | Q00535(Human); P49615(Mouse); Q03114(Rat) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of CDK5 expression in NIH3T3 (A), Hela (B), PC12 (C), COS7 (D), Jurkat (E), K562 (F) whole cell lysates. (Predicted band size: 33 kD; Observed band size: 36 kD)
Immunofluorescent analysis of CDK5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark.
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