CD20 Rabbit Monoclonal Antibody(ARA740)

Datasheet

MSDS

Key features and details

Target: CD20
Source/Host: Rabbit
Reactivity: Human
Clonality: Monoclonal
Applications: IHC,IF/ICC,FC,IP,WB
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : ARA6527

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Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

CD20 Recombinant Rabbit Monoclonal Antibody Product Datasheet Predicted Molecular Wt: 33kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Swissprot ID: P11836 Applications: IHC IF/ICC FC IP WB Background: CD20 is a leukocyte surface antigen consisting of four transmembrane regions and cytoplasmic N- and C- termini. CD20 is expressed primarily on B cells but has also been detected on both normal and neoplastic T cells. CD20 functions as a calcium-permeable cati

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:2000-1:5000
IHC	1:1000-1:2000
IF/ICC	1:400-1:1000
FC	1:100-1:500

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit Monoclonal Antibody to CD20
Antibody Type	Primary antibody
Predicted MW	33kDa
Immunogen	A synthetic peptide corresponding to residues on Copyright(c) 2023 BioLynx the C-terminus of human CD20 was used as an immunogen.
Purification	ProA affinity purified IgG
Form/Buffer	PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.15%.
Alternative Names	CD20; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD20
Gene Symbol	MS4A1
Entrez Gene	931(Human)
Swissprot	P11836

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human appendix tissue labelling CD20 with ARA740 at 1:1,000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

CD20 was immunoprecipitated from 0.4mg of Raji whole cell lysate with ARA740 at 1:20 dilution. 
2nd Ab: 
GAR HRP for IP 1:500 
 
Lane 1: ARA740 IP in Raji whole cell lysate 
Lane 2: PBS instead of ARA740 in Raji whole cell lysate 
Lane 3: Raji whole cell lysate, 10 µg (input) 
 
Exposure: 50s

ARA740 staining CD20 in Raji cells by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:1,000) at 4°C. An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain. 
Control: PBS and secondary antibody, An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (1:500).

Overlay histogram showing Raji cells stained with ARA740(Red). The cells were fixed with 4% paraformaldehyde for 10 min. The cells were then incubated in the antibody (ARA740, 1:500 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature . Unlabelled sample (Black) was used as a control.

All lanes : Anti-CD20 antibody at 1:2500 dilution 
Predicted MW: 33 kDa 
Observed MW: 33 kDa 
Lane 1 : Raji 
Lane 2 : Ramos 
Lane 3 : Daudi
 
Lysates/proteins at 10 µg per lane. Lysate at 10 µg per lane 
2nd Ab: G&R HRP(H+L) 1:5,000 
Exposure: 60s

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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