CD177 Mouse Monoclonal Antibody(C2592)

MSDS

Key features and details

Mouse monoclonal to CD177

Target: CD177
Source/Host: Mouse
Reactivity: Human
Clonality: Monoclonal
Applications: WB, FC
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : AMA02204

US$ Please choose

Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF primed neutrophils including degranulation and superoxide production . In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration . Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro . However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo . Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils .

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
FC	1:100 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Mouse monoclonal to CD177
Specificity	Recognizes endogenous levels of CD177 protein
Antibody Type	Primary antibody
Imnunogen	Recombinant fusion protein of human CD177 expressed in E. Coli
Purification	This antibody is purified through a protein G column.
Molecular Weight	Predicted: 46 kD; Observed: 46 kD kD
Form/Buffer	Mouse IgG2b. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Names	NB1; PRV1; CD177 antigen; Human neutrophil alloantigen 2a; HNA-2a; NB1 glycoprotein; NB1 GP; Polycythemia rubra vera protein 1; PRV-1; CD177
Gene Symbol	CD177
Entrez Gene	57126(Human)
SwissProt	Q8N6Q3(Human)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of CD177 expression in SPCA1 (A), SKMES1 (B), HepG2 (C), HL60 (D), PC3 (E) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 46 kD kD)

Flow cytometric analysis of HL60 cells using Anti-CD177 Antibody (green) and negative control (red).

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for use in diagnostic procedures.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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