CD154 Mouse Monoclonal Antibody(M91)

MSDS

Key features and details

Mouse monoclonal antibody to CD154

Target: CD154
Source/Host: Mouse
Reactivity: Human
Clonality: Monoclonal
Applications: WB, IF/ICC, FC
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : AMA03821

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Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Cytokine that acts as a ligand to CD40/TNFRSF5 . Costimulates T-cell proliferation and cytokine production . Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation . Induces the activation of NF-kappa-B . Induces the activation of kinases MAPK8 and PAK2 in T-cells . Induces tyrosine phosphorylation of isoform 3 of CD28 . Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 . Involved in immunoglobulin class switching .

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IF/ICC	1:50 - 1:200
FC	1:500 - 1:2000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Mouse monoclonal antibody to CD154
Specificity	Recognizes human CD154
Antibody Type	Primary antibody
Imnunogen	Native purified human CD154.
Purification	The antibody was purified by affinity chromatography.
Molecular Weight	Predicted: 29 kD; Observed: 36 kD
Form/Buffer	Mouse IgG1. Liquid in PBS, pH 7.3, and 0.02% sodium azide.
Alternative Names	CD40L; TNFSF5; TRAP; CD40 ligand; CD40-L; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD antigen CD154)
Gene Symbol	CD40LG
Entrez Gene	959(Human)
SwissProt	P29965(Human)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of CD154 expression in A2780 (A), U87 (B), A375 (C) whole cell lysates. (Predicted band size: 29 kD; Observed band size: 36 kD)

Flow cytometric analysis of PBMC cells treated with PHA and lonomycin for 5 hours using Anti-CD154 Antibody, followed by anti-mouse IgG PE.

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for use in diagnostic procedures.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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