CD14 Mouse Monoclonal Antibody(3C10)
MSDS 
Key features and details
Mouse monoclonal antibody to CD14
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
-
Brand:
CAT.NO. : AMA03713
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Product Details
Background
Coreceptor for bacterial lipopolysaccharide . In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) . Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response . Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway . Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) .
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:200 |
FC | 1:500 - 1:2000 |
Overview
Description | Mouse monoclonal antibody to CD14 |
Specificity | Recognizes human CD14 |
Antibody Type | Primary antibody |
Imnunogen | Native purified human CD14. |
Purification | The antibody was purified by affinity chromatography. |
Molecular Weight | Predicted: 40 kD; Observed: 53 kD |
Form/Buffer | Mouse IgG2b. Liquid in PBS, pH 7.3, and 0.02% sodium azide. |
Alternative Names | Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD antigen CD14) |
Gene Symbol | CD14 |
Entrez Gene | 929(Human) |
SwissProt | P08571(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Western blot analysis of CD14 expression in mouse lung (A) whole cell lysates. (Predicted band size: 40 kD; Observed band size: 53 kD)
Flow cytometric analysis of human peripheral blood monocytes using Anti-CD14 Antibody, followed by anti-mouse IgG PE.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for use in diagnostic procedures.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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