CARS Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:200 |
Description | Rabbit polyclonal antibody to CARS |
Specificity | Recognizes endogenous levels of CARS protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human CARS |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 82; Observed: 85 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | Cysteine--tRNA ligase, cytoplasmic; Cysteinyl-tRNA synthetase; CysRS |
Gene Symbol | CARS |
Entrez Gene | 833(Human) |
SwissProt | P49589(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of CARS expression in Hela (A), MCF7 (B), mouse liver (C), rat spinal cord (D) whole cell lysates. (Predicted band size: 82; 85; 94 kD; Observed band size: 85 kD)
Immunofluorescent analysis of CARS staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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