c-SRC (Phospho-Y419) Rabbit Polyclonal Antibody
Key features and details
- Reactivity:Human, Rat, Mouse, Bovine, Chicken, Pig, Zebrafish
- Host:Rabbit
- Clonality:Polyclonal
- Application:WB
- Target:c-SRC (Phospho-Y419)
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Brand:
CAT.NO. : APA19560
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Product Details
Background
SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. SRC is involved in the regulation of growth and differentiation of eukaryotic cells. SRC associates with the cytoplasmic region of various cell surface receptors to facilitate signal transduction, and is also involved in cytoskeletal organization, cell cycle regulation, and apoptosis. In humans, the gene encoding SRC is present on chromosome 20. Activity of SRC is regulated by two tyrosine residues (Tyr 416 and Tyr527), and phosphorylation of these two sites have opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates enzyme activity while the phosphorylation of Tyr527 decreases enzyme activity. Mutations in the SRC gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same SRC protein have been found thus far.
Aplication
|
Application |
Dilution Ratio |
|
WB |
1:500 - 1:1000 |
Overview
|
Product Description |
Rabbit polyclonal antibody to c-SRC (Phospho-Y419) |
|
Immunogen |
KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y419 of human c-SRC protein. The exact sequence is proprietary. |
|
Purification Method |
The antibody was purified by immunogen affinity chromatography. |
|
Clonality |
Polyclonal |
|
Form |
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
|
Gene Symbol |
SRC |
|
Alternative Names |
SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src |
|
Gene ID (Human) |
6714 |
|
Gene ID (Mouse) |
20779 |
|
Gene ID (Rat) |
83805 |
|
Protein ID (Human) |
P12931 |
|
Protein ID (Mouse) |
P05480 |
|
Protein ID (Rat) |
Q9WUD9 |
Data
Western blot analysis of c-SRC (Phospho-Y419) expression in HEK293T EGF-treated (A), COLO205 (B), Raw264.7 EGF-treated (C), PC12 EGF-treated (D) whole cell lysates. (Predicted band size: 59 kD; Observed band size: 60 kD)
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for use in diagnostic procedures.
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