Beta III tubulin Rabbit Monoclonal Antibody(ARA733)

Datasheet

MSDS

Beta III tubulin Rabbit Monoclonal Antibody(ARA733)

Key features and details

Target: Beta III tubulin
Source/Host: Rabbit
Reactivity: Human, Rat, Mouse
Clonality: Monoclonal
Applications: WB,FC
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : ARA6520

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Size:

100μL

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Product Details

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:2000-1:5000
FC	1:200-1:1000
IP	1:10

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit Monoclonal Antibody to Beta III tubulin
Antibody Type	Primary antibody
Predicted MW	50kDa
Immunogen	A synthetic peptide corresponding to residues on the C-terminus of human beta III tubulin was used as an immunogen.
Purification	ProA affinity purified IgG
Form/Buffer	PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.08%.
Alternative Names	TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III
Gene Symbol	TUBB3
Entrez Gene	10381(Human); 22152(Mouse); 246118(Rat)
Swissprot	Q13509

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

All lanes: Anti-Beta III tubulin antibody at 1:5,000 dilution
Predicted MW: 50 kDa
Observed MW: 50 kDa

Lane 1: Jurkat
Lane 2: PC-12
Lane 3: 3T3

Lysates at 10 μg per lane
2nd Ab:
GAR HRP(H+L) 1:10,000
Exposure: 40s

All lanes: Anti-Beta III tubulin antibody at 1:5,000 dilution
Predicted MW: 50 kDa
Observed MW: 50 kDa

Lane 1: Mu Heart
Lane 2: Mu Kidney
Lane 3: Mu Liver
Lane 4: Rat Heart
Lane 5: Rat Liver

Lysates at 10 μg per lane
2nd Ab:
GAR HRP(H+L) 1:10,000
Exposure: 100s

Overlay histogram showing Hela cells stained with ARA733 (gold). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the antibody (ARA733, 1:1,000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature. Unlabelled sample (Red) was used as a control.

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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