BAX Rabbit Monoclonal Antibody(ARA762)

Datasheet

MSDS

Key features and details

Target: BAX
Source/Host: Rabbit
Reactivity: Human, Mouse, Rat
Clonality: Monoclonal
Applications: WB,FC,IP
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : ARA6549

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Size:

100μL

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Product Details

Background

BAX Recombinant Rabbit Monoclonal Antibody Product Datasheet Predicted Molecular Wt: 21kDa Purity: ProA affinity purified IgG Species Cross‐reactivity: Human Mouse Rat Form: Liquid Species cross‐reactivity determined by WB Swissprot ID: Q07812 Applications: WB IHC‐P FC IP Background: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes transloc

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500-1:1000
FC	1:50-1:100
IP	1:25

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit Monoclonal Antibody to BAX
Antibody Type	Primary antibody
Predicted MW	21kDa
Immunogen	Synthetic peptide according to the aa 1‐100 (C terminus) of BAX was used as an immunogen.
Purification	ProA affinity purified IgG
Form/Buffer	PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.37%.
Alternative Names	BCL2L4; Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4
Gene Symbol	BAX
Entrez Gene	581(Human)
Swissprot	Q07812

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

All lanes: Anti-BAX antibody at 1:1,000 dilution
Predicted MW: 21 kDa
Observed MW: 18 kDa

Lane 1: OVCAR-3
Lane 2: A549
Lane 3: HT-29
Lane 4: SH-SY5Y
Lane 5: LnCap

Lysates at 10 µg per lane
2nd Ab:
GAR HRP(H+L) 1:10,000
Exposure: 120s

All lanes: Anti-BAX antibody at 1:1,000 dilution
Predicted MW: 21 kDa
Observed MW: 18 kDa

Lane 1: Mouse Kidney
Lane 2: Rat Brain
Lane 3: Rat Kidney
Lane 4: Rat Liver

Lysates at 10 µg per lane
2nd Ab:
GAR HRP(H+L) 1:10,000
Exposure: 120s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian cancer tissue labelling BAX with ARA762 at 1:1,600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Overlay histogram showing Hela cells stained with ARA762 (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the antibody (ARA762, 1:400 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor<sup>®</sup> 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.

BAX was immunoprecipitated from 0.5mg HT-29 whole cell lysate with ARA762 at 1:25 dilution.
2nd Ab:
GAR HRP for IP 1:500
1. ARA762 IP in HT-29 whole cell lysate
2. PBS instead of ARA762 in HT-29 whole cell lysate
3. HT-29 whole cell lysate, 10 µg (input)
Exposure:30s

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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