AREX® Fluor 594 Rabbit IgG(H+L) Multiclonal Goat Monoclonal antibody

Key features and details

  • Target: Rabbit IgG(H+L)
  • Host: Goat
  • Reactivity: Rabbit
  • Clonality: Multiclonal
  • Application: IF/ICC, FC
  • Conjugate: AREX® Fluor 594
  • Storage: -20°C
  • Brand:
CAT.NO. : ARA6456
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Size:
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Product Details
Background
AREX Multiclonal Monoclonal Antibodies are precision-engineered formulations of recombinant monoclonal antibodies, each targeting distinct complementary epitopes on the same IgG molecule. Every recombinant clone included in the final multiclonal mixture undergoes stringent characterization and screening to ensure exceptional functional performance. Candidate clones are then combined in varying ratios, with each combination functionally validated across multiple experimental applications to identify the optimal formulation. Critically, each production lot of AREX Multiclonal Monoclonal Antibody uses the identical set of high-performance recombinant clones, ensuring exceptional batch-to-batch consistency and highly reproducible experimental results.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application

Dilution Ratio

IF/ICC

1:200-1:1000

FC

1:200-1:1000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Overview

Host Species

Goat

Species Reactivity

Rabbit

Physical State

Liquid

Purity

The antibody was purified from culture media supernatant by immunoaffinity chromatography using Protein G beads.

Form/Buffer

PBS with 50% glycerol, 10 mg/mL BSA, 0.1% Proclin-300, pH 7.4.

Fluorophore

AREX® Fluor 594, Amax=588 nm, Emax=604 nm

Clonality

Multiclonal

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data

Immunofluorescence of rat brain: rat brain FFPE section was stained with GFAP Rabbit polyclonal antibody (red) and NeuN Mouse monoclonal antibody (green). AREX® Fluor 594 Rabbit IgG(H+L) Multiclonal Goat Monoclonal antibody and AREX® Fluor 488 Mouse IgG(H+L) Multiclonal Goat Monoclonal antibody were used for detection .

1X10^6 MOLT4 were surface stained with 0.2 ug HLA class I rabbit polyclonal antibody and Rabbit IgG Isotype Control. AREX® Fluor 594 Rabbit IgG(H+L) Multiclonal Goat Monoclonal antibody was used at 1:500 for detection.

Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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