Anti-SARS-CoV-2 Spike Protein Mouse Monoclonal Antibody (G5)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
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Brand:
CAT.NO. : AXA0001
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Size:
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Product Details
Background
The spike protein (S protein) of SARS-CoV-2 is a key structural protein on the surface of the novel coronavirus, responsible for recognizing the host cell receptor ACE2 and mediating the membrane fusion process. The S protein exists as a trimer, with each monomer containing approximately 1300 amino acids and consisting of two subunits, S1 and S2. The receptor-binding domain (RBD) of the S1 subunit is responsible for binding to ACE2, while the S2 subunit promotes membrane fusion through conformational changes.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
WB | 1:500-1:1000 |
IF/ICC | 1:500-1:1000 |
ELISA | 1:2000-1:10000 |
Overview
Description | Mouse Monoclonal antibody to SARS-CoV-2 Spike Protein |
Antibody Type | Primary antibody |
Immunogen | SARS-CoV-2 S Protein |
Form/Buffer | PBS, 20% Glycerol; preservative: 0.05% Sodium Azide |
Alternative Names | SARS-CoV-2 S, COVID-19 spike protein, 2019-nCoV Spike, SARS-CoV-2 S glycoprotein, Spike RBD/S1/S2 |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
ELISA results indicate that this antibody can effectively recognize the S2 antigen polypeptide.
WB results demonstrate that this antibody can effectively recognize the SARS - CoV - 2 S protein expressed in 293T cells.
IFA results demonstrate that this antibody can effectively recognize the SARS - CoV - 2 S protein expressed in 293T cells.
Reference
1. Li T, Kan Q, Ge J, et al. A novel linear and broadly neutralizing peptide in the SARS-CoV-2 S2 protein for universal vaccine development. Cell Mol Immunol. 2021;18(11):2563-2565. doi:10.1038/s41423-021-00778-6.
2. Kan Q, Lin X, Li T, et al. A novel mAb broadly neutralizes SARS-CoV-2 VOCs in vitro and in vivo, including the Omicron variants. J Med Virol. 2023;95(3):e28657. doi:10.1002/jmv.28657.
2. Kan Q, Lin X, Li T, et al. A novel mAb broadly neutralizes SARS-CoV-2 VOCs in vitro and in vivo, including the Omicron variants. J Med Virol. 2023;95(3):e28657. doi:10.1002/jmv.28657.
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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