Acinus (Phospho-S1180) Rabbit Polyclonal Antibody

MSDS

Acinus (Phospho-S1180) Rabbit Polyclonal Antibody

Key features and details

Rabbit polyclonal antibody to Acinus (Phospho-S1180)

Target: Acinus (Phospho-S1180)
Source/Host: Rabbit
Reactivity: Human, Mouse, Rat
Clonality: Polyclonal
Applications: WB
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : APA10711

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Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets; ACIN1 confers RNA-binding to the complex. The ASAP complex can inhibit RNA processing during in vitro splicing reactions.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit polyclonal antibody to Acinus (Phospho-S1180)
Specificity	Recognizes endogenous levels of Acinus protein only when phosphorylated at S1180.
Antibody Type	Primary antibody
Imnunogen	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S1180 of human Acinus protein. The exact sequence is proprietary.
Purification	The antibody was purified by immunogen affinity chromatography.
Molecular Weight	Predicted: 151; Observed: 210 kD
Form/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Names	ACIN1; ACINUS; KIAA0670; Apoptotic chromatin condensation inducer in the nucleus; Acinus; SCAF4; KIAA1172; SFRS15; Splicing factor arginine/serine-rich 15; CTD-binding SR-like protein RA4; SR-related and CTD-associated factor 4; PNISR; C6orf111; SFRS18; SRRP130; HSPC261; HSPC306; Arginine/serine-rich protein PNISR; PNN-interacting serine/arginine-rich protein; SR-related protein; SR-rich protein; Serine/arginine-rich-splicing regulatory protein 130; SRrp130; Splicing factor arginine/serine-rich 130; Splicing factor arginine/serine-rich 18; RBM39; HCC1; RNPC2; RNA-binding protein 39; Hepatocellular carcinoma protein 1; RNA-binding motif protein 39; RNA-binding region-containing protein 2; Splicing factor HCC1
Gene Symbol	ACIN1
Entrez Gene	22985; 57466; 25957; 9584(Human); 56215; 170791(Mouse)
SwissProt	Q9UKV3; O95104; Q8TF01; Q14498(Human); Q9JIX8; A2AJT4; Q8VH51(Mouse); Q63627(Rat)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of Acinus (Phospho-S1180) expression in A549 (A), Hela (B), Jurkat (C), NIH3T3 (D), H9C2 (E), PC12 (F) whole cell lysates. (Predicted band size: 151; 125; 92; 59 kD; Observed band size: 210 kD)

Direct ELISA antibody dose-response curve using Anti-Acinus (Phospho-S1180) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for use in diagnostic procedures.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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