5-HT7 Rabbit Polyclonal Antibody
Key features and details
- Reactivity:
- Application:
- Host:
- Clonality:
- Target:
-
Brand:
CAT.NO. : ARA6833
US$ Please choose
US$ Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
5-HT7 Receptor is a Serotonin Receptor with several functions, including regulation of the circadian rhythm and mediation of smooth-muscle relaxation in the vasculature and GI tract. This receptor has been implicated in depression, autism, and other neuropsychiatric disorders. Three protein isoforms have been documented: isoform a: 445aa; isoform b: 432aa; isoform d: 479aa. 5-HT7 Receptor has been reported in aorta, brain, heart, placenta, small intestine, spleen, artery, and vessel. An EST has been isolated from a testis library.
Application
|
Application |
Dilution Ratio |
|
WB |
1:500 - 1:1000 |
|
IF/ICC |
1:50 - 1:200 |
Overview
|
Immunogen |
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human 5-HT7. The exact sequence is proprietary |
|
Purification Method |
The antibody was purified by immunogen affinity chromatography |
|
Clonality |
Polyclonal |
|
Form |
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide |
|
Gene Name |
HTR7 |
|
Synonyms |
5-hydroxytryptamine receptor 7; 5-HT7; 5-HT7; 5-HT-X; Serotonin receptor 7 |
|
Gene ID (Human) |
3363 |
|
Gene ID (Mouse) |
15566 |
|
Gene ID (Rat) |
65032 |
|
Protein ID (Human) |
P34969 |
|
Protein ID (Mouse) |
P32304 |
|
Protein ID (Rat) |
P32305 |
Data
Western blot analysis of 5-HT7 expression in Hela (A), H1688 (B) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 54 kD)
Immunofluorescent analysis of 5-HT7 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
New Products
