Stathmin Rabbit Monoclonal Antibody(ARB554)
Datasheet 
Key features and details
- Target:
- Clone ID:
- Source/Host:
- Reactivity:
- Applications:
- Dilution:
- Clonality:
- Storage:
-
Brand:
CAT.NO. : ARB6846
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Size:
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Product Details
Background
Stathmin/oncoprotein 18, also known as STMN1, performs an important function in regulating rapid microtubule remodeling of the cytoskeleton in response to the cell’s needs. Regulation of stathmin is cell cycle dependent and controlled by the cell’s protein kinases in response to specific cell signals. Stathmin can cause uncontrolled cell proliferation when mutated and not functioning properly.Overexpression of Stathmin has been associated with tumor progression in endometrial carcinomas, ovarian cancer and oral squamous-cell carcinoma. Stathmin has been found to be positive in 29% of cervical intraepithelial neoplasia (CINs) with differential expression based on the grade of the lesion as 10% CIN1, 45% CIN2, and 93% CIN3; whereas, p16 staining of the same cases was positive in 80% of CINs with 71% CIN1, 90% CIN2, and 94% CIN3. Stathmin shows similar sensitivity for CIN3 to anti-p16 (93% vs 94%) although it drops off for CIN2 (73% vs 96%). The specificity of stathmin antibody for both CIN2-3 (94%) and CIN3 (89%) is higher than that of anti-p16 (44% and 39%, respectively). Therefore, Stathmin antibody has major potential as a diagnostic marker in CIN classification over anti-p16 and it is valuable to distinguish CIN3 from the majority of low-grade precursors and negative/reactive cervical biopsies.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
IHC | 1:100 - 1:200 |
Overview
Predicted Molecular Wt | 17kDa |
Purity | ProA affinity purified IgG |
Subcellular location | Membrane/cytoplasm |
Swissprot ID | P16949 |
Immunogen | Synthetic peptide. |
Storage Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
Recommended Method | Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18℃-25℃) for 30 minutes |
Alternative Names | Oncoprotein 18, Op18 |
Gene Symbol | STMN1 |
Entrez Gene | 3925 |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Immunohistochemical staining of human tonsil tissue using Stathmin Rabbit Monoclonal Antibody(ARB554).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?
AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.
What development scenarios are pathology raw antibodies mainly used for?
Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.
How can I evaluate whether a raw antibody is suitable for our staining platform?
It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.
Can you provide samples for platform validation?
Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.
How should raw antibodies be paired with detection systems?
AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.
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