SREBP-1c Rabbit Polyclonal Antibody
Key features and details
- Reactivity:
- Application:
- Host:
- Clonality:
- Target:
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Brand:
CAT.NO. : ARA6804
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Product Details
Background
Sterol regulatory element-binding transcription factor 1 (SREBF1) also known as sterol regulatory element-binding protein 1 (SREBP-1) is a protein that in humans is encoded by the SREBF1 gene. This gene is located within the Smith–Magenis syndrome region on chromosome 17. Two transcript variants encoding different isoforms have been found for this gene. The isoforms are SREBP-1a and SREBP-1c (the latter also called ADD-1). SREBP-1a is expressed in the intestine and spleen, whereas SREBP-1c is mainly expressed in liver, muscle, and fat (among other tissues). SREBP-1 plays a key role in the induction of lipogenesis by the liver. mTORC1 is activated by insulin (a hormone of nutrient abundance) leading to increased production of SREBP-1c, which facilitates storage of fatty acids (excess nutrients) as triglycerides.
Application
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Application |
Dilution Ratio |
|
WB |
1:500 - 1:1000 |
Overview
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Immunogen |
Recombinant protein corresponding to human SREBP-1c. The exact sequence is proprietary |
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Purification Method |
The antibody was purified by immunogen affinity chromatography |
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Clonality |
Polyclonal |
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Form |
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide |
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Gene Name |
SREBF1 |
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Synonyms |
BHLHD1; SREBP1; Sterol regulatory element-binding protein 1; SREBP-1; Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1 |
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Gene ID (Human) |
6720 |
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Gene ID (Mouse) |
20787 |
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Gene ID (Rat) |
78968 |
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Protein ID (Human) |
P36956 |
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Protein ID (Mouse) |
Q9WTN3 |
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Protein ID (Rat) |
P56720 |
Data
Western blot analysis of SREBP-1c expression in mouse kidney (A), rat brain (B) whole cell lysates. (Predicted band size: 45; 48; 111; 113; 121; 124 kD; Observed band size: 125 kD)
Immunohistochemical analysis of SREBP-1c staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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