SP1 Mouse Monoclonal Antibody(C3634)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:10 - 1:50 |
FC | 1:10 - 1:50 |
Description | Mouse monoclonal antibody to SP1 |
Specificity | Recognizes endogenous levels of SP1 protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human SP1. The exact sequence is proprietary. |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 80 kD; Observed: 105 kD |
Form/Buffer | Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | TSFP1; Transcription factor Sp1 |
Gene Symbol | SP1 |
Entrez Gene | 6667(Human) |
SwissProt | P08047(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of SP1 expression in Hela (A), Ramos (B) whole cell lysates. (Predicted band size: 80 kD; Observed band size: 105 kD)
Immunofluorescent analysis of SP1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of Hela cells using Anti-SP1 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AREX® Fluor 488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
Western blot analysis of SP1 expression in wild type (WT) and knockdown (KD) HeLa cell lysates.
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