Serum-free Medium for Exosome (Phenol red-free)
Protocol Booklet 
Key features and details
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CAT.NO. : UR51113
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Product Details
Background
Serum‑free medium for exosomes (phenol red‑free) is a medium specifically designed for exosome‑related applications. The product contains HEPES, L‑Glutamine, Pharm Grade Albumin, Hypoxanthine, Thymidine, and Glucose. It is Xeno‑free and Serum‑free, and does not contain growth factors. It can be used as a complete medium without the addition of serum during cell culture, which is conducive to exosome harvesting. Phenol red is commonly used in culture media as a pH indicator to continuously monitor the acidity/alkalinity of the culture solution. However, phenol red also has certain drawbacks. Studies have shown that phenol red can mimic the effects of steroid hormones (especially estrogen), so it is recommended to use phenol red‑free medium when working with estrogen‑sensitive cells (e.g., mammary tissue). In addition, under certain conditions, phenol red may interfere with sodium‑potassium balance and with flow cytometry analysis in cell labeling experiments. This medium does not contain phenol red and is free from the above‑mentioned effects. Applicable cells: Suitable for both tumor cells and T cells.
Features
1.Free from phenol red interference
2.Medium specifically designed for exosomes; Xeno-free and serum-free
3.Wide applicability with reliable and consistent results
2.Medium specifically designed for exosomes; Xeno-free and serum-free
3.Wide applicability with reliable and consistent results
Advantages of the Product
1.Focus on exosome isolation and the R&D of supporting kits.
2.Backed by hundreds of references for your confident use and worry-free purchase.
3.Medium specifically designed for exosomes.
4.Xeno-free, serum-free and phenol red-free.
5.Wide applicability with reliable and consistent results.
2.Backed by hundreds of references for your confident use and worry-free purchase.
3.Medium specifically designed for exosomes.
4.Xeno-free, serum-free and phenol red-free.
5.Wide applicability with reliable and consistent results.
Data
These charts/images include the following sections (taking HeLa cells as an example):
1.Spheroid formation comparison results
Group A: Regular medium
Group B: Specialized medium (Displayed via spheroid morphology images of the two groups)
2.Electron microscopy identification results
Group A: Non-exosome serum culture group
Group B: Regular serum medium group
Group C: Exosome-specialized culture group (Presented through electron microscopy images of the three groups)
3.NTA (Nanoparticle Tracking Analysis) test results (Showed via NTA detection curve plots)
Storage
2-8℃
Shipping temp
cold temperature
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