SAF-B1 Mouse Monoclonal Antibody(C3324)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:100 |
ChIP | 1:10 - 1:50 |
Description | Mouse monoclonal antibody to SAF-B1 |
Specificity | Recognizes endogenous levels of SAF-B1 protein. |
Antibody Type | Primary antibody |
Imnunogen | Purified recombinant human SAFB1 protein fragments expressed in E.coli. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 103 kD; Observed: 130 kD |
Form/Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
Alternative Names | HAP; HET; SAFB1; Scaffold attachment factor B1; SAF-B; SAF-B1; HSP27 estrogen response element-TATA box-binding protein; HSP27 ERE-TATA-binding protein |
Gene Symbol | SAFB |
Entrez Gene | 6294(Human) |
SwissProt | Q15424(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of SAF-B1 expression in Hela (A), A431 (B), MDAMB468 (C), ZR751 (D), MCF7 (E) whole cell lysates. (Predicted band size: 103 kD; Observed band size: 130 kD)
Immunofluorescent analysis of SAF-B1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark.
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