S100P Rabbit Monoclonal Antibody(ARA751)
Datasheet 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
IHC | 1:200-1:400 |
IF/ICC | 1:1000-1:2000 |
FC | 1:10-1:50 |
Description | Rabbit Monoclonal Antibody to S100P |
Antibody Type | Primary antibody |
Predicted MW | 10kDa |
Immunogen | A synthetic peptide corresponding to residues on the C-terminus of human S100P was used as an Copyright(c) 2017 BioLynx immunogen. |
Purification | ProA affinity purified IgG |
Form/Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.26%. |
Alternative Names | S100E; Protein S100-P; Migration-inducing gene 9 protein; MIG9; Protein S100-E; S100 calcium-binding protein P |
Gene Symbol | S100P |
Entrez Gene | 6286(Human) |
Swissprot | P25815 |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling S100P with ARA751 at 1:200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling S100P with ARA751 at 1:200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
ARA751 staining S100P in HT-29 cells by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:2,000) at 4°C. An Alexa Fluor<sup>®</sup> 594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An Alexa Fluor<sup>®</sup> 594-conjugated Goat Anti-Rabbit IgG (1:500).
Overlay histogram showing BxPC-3 cells stained with ARA751 (Blue). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the antibody (ARA751, 1:50 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor<sup>®</sup> 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature. Unlabelled sample (Red) was used as a control.
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