RNF10 Rabbit Polyclonal Antibody
MSDS 
Key features and details
Rabbit polyclonal antibody to RNF10
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
CAT.NO. : APA16729
US$ Please choose
US$ Please choose
Product Details
Background
E3 ubiquitin-protein ligase that catalyzes monoubiquitination of 40S ribosomal proteins RPS2/us5 and RPS3/us3 in response to ribosome stalling . Part of a ribosome quality control that takes place when ribosomes have stalled during translation initiation (iRQC) or elongation . The ribosome quality control is activated in response to ribosome subunit imbalance, amino acid starvation or downstream the EIF2AK4/GCN2-mediated integrated stress response (ISR) . RNF10 acts by mediating monoubiquitination of RPS2/us5 and RPS3/us3: monoubiquitinated RPS2/us5 and RPS3/us3 are then recognized by RIOK3 kinase, leading to 18S non-functional rRNA decay and degradation of the 40S ribosomal subunit . The action of RNF10 in iRQC is counteracted by USP10 .
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:1000 |
Overview
Description | Rabbit polyclonal antibody to RNF10 |
Specificity | Recognizes endogenous levels of RNF10 protein |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human RNF10. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 89; Observed: 100 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | KIAA0262; RIE2; RING finger protein 10 |
Gene Symbol | RNF10 |
Entrez Gene | 9921(Human); 50849(Mouse); 288710(Rat) |
SwissProt | Q8N5U6(Human); Q3UIW5(Mouse); Q5XI59(Rat) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Western blot analysis of RNF10 expression in Raji (A), mouse brain (B) whole cell lysates. (Predicted band size: 89; 90 kD; Observed band size: 100 kD)
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for use in diagnostic procedures.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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