RIPK1 Rabbit Monoclonal Antibody(YJY-5-8)
Datasheet 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
-
Brand:
CAT.NO. : ARA6604
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Size:
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Product Details
Background
Serine-threonine kinase which is a key regulator of TNF-mediated apoptosis, necroptosis and inflammatory pathways . Exhibits kinase activity-dependent functions that regulate cell death and kinase-independent scaffold functions regulating inflammatory signaling and cell survival . Has kinase-independent scaffold functions: upon binding of TNF to TNFR1, RIPK1 is recruited to the TNF-R1 signaling complex (TNF-RSC also known as complex I) where it acts as a scaffold protein promoting cell survival, in part, by activating the canonical NF-kappa-B pathway .
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500-1:2000 |
IHC | 1:10-1:100 |
Overview
Description | Rabbit Monoclonal Antibody to RIPK1 |
Antibody Type | Primary antibody |
Predicted MW | 75kDa |
Immunogen | A synthetic peptide corresponding to residues aa200- 300 of human RIPK1 was used as an immunogen. |
Purification | ProA affinity purified IgG |
Form/Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.96%. |
Alternative Names | RIP; RIP1; Receptor-interacting serine/threonine-protein kinase 1; Cell death protein RIP; Receptor-interacting protein 1; RIP-1; Serine/threonine-protein kinase RIP |
Gene Symbol | RIPK1 |
Entrez Gene | 8737(Human) |
Swissprot | Q13546 |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
All lanes: Anti-Human RIPK1 antibody at 1:2000 dilution
Predicted MW: 75 kDa
Observed MW: 70 kDa
Lane 1: WT Jurkat cell lysates
Lane 2: RIPK1 deficient Jurkat cell lysates
Lysates at approx. 10 µg per lane
2nd Ab: GAR HRP(H+L) 1:4,000
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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