Rat IL-4 ARExSet® antibody pair for ELISA
Protocol Booklet 
Key features and details
- Species:
- Size:
- Sample Volume:
- Standard Curve Range:
- Assay type:
- Sensitivity:
- Operation Duration:
- Assay type:
-
Brand:
CAT.NO. : ASER0003
US$ Please choose
US$ Please choose
Product Details
Background
Interleukin 4 (IL-4)
A cytokine that plays a role in many immunologic processes, including allergic inflammation and asthma:
Production: IL-4 is primarily produced by mast cells, eosinophils, basophils, and T helper 2 (Th2) cells.
Function: IL-4 induces the differentiation of naive helper T cells into Th2 cells, which then produce more IL-4. IL-4 also regulates immunoglobulin class switching in B cells.
Clinical significance: IL-4 receptors are overexpressed in many types of cancer and are being studied as potential cancer biomarkers and therapeutic targets. IL-4 is also elevated in patients with cardiovascular disease.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0371 | 0.0392 | 0.0382 | |
| 15.63 | 0.1070 | 0.1233 | 0.1152 | 0.0102 |
| 31.25 | 0.1806 | 0.1997 | 0.1902 | 0.0435 |
| 62.50 | 0.3138 | 0.3652 | 0.3395 | 0.1166 |
| 125.00 | 0.6154 | 0.6110 | 0.6132 | 0.2566 |
| 250.00 | 1.0940 | 1.2750 | 1.1845 | 0.6392 |
| 500.00 | 1.9630 | 1.9840 | 1.9735 | 1.7546 |
| 1000.00 | 3.2590 | 3.3080 | 3.2835 | 3.8611 |
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
ARExSet® Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.
- 96 well microplates: AREX Biosciences, Catalog # DSEP01. Plate Sealers: AREX Biosciences, Catalog # DSSF01.
- Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2-7.4, 0.2 μm fi ltered . AREX Biosciences, Catalog # DSCB01.
- Blocking Buffer: AREX Biosciences, Catalog # DSBB01.
- Wash Buffer: 0.05% Tween 20 in PBS, pH 7.2-7.4. AREX Biosciences, Catalog # DSWB01.
- Assay Buffer: 0.5%BSA，0.05%Tween20，PBS Solution.AREX Biosciences, Catalog # DSAB01
- Substrate Solution: Tetramethylbenzidine. AREX Biosciences, Catalog # DSTS01.
- Stop Solution: 0.5mol/ml H2SO4. AREX Biosciences, Catalog # DSSS01.
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