RAB11B-Specific Rabbit Polyclonal Antibody

Datasheet

MSDS

RAB11B-Specific Rabbit Polyclonal Antibody

Key features and details

Target: RAB11B-Specific
Host: Rabbit
Reactivity: Human, Mouse, Rat
Clonality: Polyclonal
Application: WB, IHC, IP, ELISA
Storage: -20°C

Brand:

CAT.NO. : ARA6367

US$ Please choose

Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

RAB11B, also named as YPT3, belongs to the small GTPase superfamily and Rab family. RAB11B possesses GTPase activity. The antibody is specific to RAN11B. The antibody has no cross reaction to RAB11A.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:200-1:1000
IHC	1:20-1:200
IP	0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Recommended Method	WB, IP, IHC, ELISA
Reactivity Species	Human, Mouse, Rat, Canine
Immunogen	Peptide
Host Species / Isotype	Rabbit / IgG
Clonality	Polyclonal
Product Type	Antibody
Alternative Names	GTP binding protein YPT3, H YPT3, RAB11B, RAB11B-Specific, Ras related protein Rab 11B, YPT3
Molecular Weight	24 kDa
Gene Symbol	RAB11B
Gene ID (Human)	9230
Conjugate	Unconjugated
Form	Liquid

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

PC-12 cells were subjected to SDS PAGE followed by western blot with RAB11B-Specific Rabbit Polyclonal Antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human brain using RAB11B-Specific Rabbit Polyclonal Antibody at dilution of 1:50 .

IP result of RAB11B-Specific Rabbit Polyclonal Antibody (IP:RAB11B-Specific Rabbit Polyclonal Antibody, 4ug; Detection:RAB11B-Specific Rabbit Polyclonal Antibody 1:1000) with mouse brain tissue lysate 3200 ug.

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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