Phospholipase D2 (Phospho-Y169) Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IHC | 1:50 - 1:100 |
Description | Rabbit polyclonal antibody to Phospholipase D2 (Phospho-Y169) |
Specificity | Recognizes endogenous levels of Phospholipase D2 protein only when phosphorylated at Y169. |
Antibody Type | Primary antibody |
Imnunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y169 of human Phospholipase D2 protein. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 105 kD; Observed: 105 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | Phospholipase D2; PLD 2; hPLD2; Choline phosphatase 2; PLD1C; Phosphatidylcholine-hydrolyzing phospholipase D2 |
Gene Symbol | PLD2 |
Entrez Gene | 5338(Human); 18806(Mouse); 25097(Rat) |
SwissProt | O14939(Human); P97813(Mouse); P70498(Rat) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of Phospholipase D2 (Phospho-Y169) expression in HEK293T TNFa-treated (A), NIH3T3 TNFa-treated (B), H9C2 TNFa-treated (C) whole cell lysates. (Predicted band size: 105 kD; Observed band size: 105 kD)
Immunohistochemical analysis of Phospholipase D2 (Phospho-Y169) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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