Phospholipase D Activity Fluorometric Microplate Assay Kit
Key features and details
- Applications:
- Alternative Names:
- CAS Number:
- Detection Method:
- Detection Range:
- Sample Types:
- Platform:
- Storage:
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Brand:
CAT.NO. : ARS6905
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Product Details
Background
Phospholipase D (PLD) catalyses the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and a free headgroup. Abnormalities in PLD expression have been associated with human cancers.
AREX provides a simple and high-throughput assay for measuring PLD activity. In this assay, PLD hydrolyzes phosphatidylcholine to choline which is determined using choline oxidase and an H2O2-specific dye.
AREX provides a simple and high-throughput assay for measuring PLD activity. In this assay, PLD hydrolyzes phosphatidylcholine to choline which is determined using choline oxidase and an H2O2-specific dye.
Overview
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Synonyms |
EC 3.1.4.4; lipophosphodiesterase II; lecithinase D; choline phosphatase |
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CAS Number |
9001-87-0 |
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Detection Method |
Fluorimetric |
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Detection Range |
1 μmol/L - 100 pmol/L |
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Sample Type |
Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids |
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Detection Platform |
Fluorescence microplate reader Ex/Em = 535/587 nm |
Data
Representative Standard Curve
Storage
Store at 4°C. Stable for 6 month from the date of shipment.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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