Periostin Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:2000 |
IHC | 1:50 - 1:200 |
IF/ICC | 1:50 - 1:200 |
Description | Rabbit polyclonal antibody to Periostin |
Specificity | Recognizes endogenous levels of Periostin protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human Periostin |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 80; Observed: 100 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | OSF2; Periostin; PN; Osteoblast-specific factor 2; OSF-2 |
Gene Symbol | POSTN |
Entrez Gene | 10631(Human) |
SwissProt | Q15063(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of Periostin expression in OVCAR3 (A), SKOV3 (B) whole cell lysates. (Predicted band size: 80; 83; 87; 90; 93 kD; Observed band size: 100 kD)
Immunohistochemical analysis of Periostin staining in human uterus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Periostin staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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