NPM1/Nucleophosmin Rabbit Monoclonal Antibody(ARA648)

MSDS

NPM1/Nucleophosmin Rabbit Monoclonal Antibody(ARA648)

Key features and details

Target: NPM1/Nucleophosmin
Host: Rabbit
Reactivity: Human
Clonality: Monoclonal
Applications: IHC
Conjugation: Unconjugated
Storage: -20°C

Brand:

CAT.NO. : ARA6355

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Size:

1mL

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Product Details

Background

Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. NPM1 gene is up-regulated, mutated and chromosomally translocated in many tumor types. Chromosomal aberrations involving NPM1 were found in patients with non-Hodgkin lymphoma, acute promyelocytic leukemia, myelodysplastic syndrome, and acute myelogenous leukemia. Heterozygous mice for NPM1 are vulnerable to tumor development. In solid tumors NPM1 is frequently found overexpressed, and it is thought that NPM1 could promote tumor growth by inactivation of the tumor suppressor p53/ARF pathway; on the contrary, when expressed at low levels, NPM1 could suppress tumor growth by the inhibition of centrosome duplication. Of high importance is NPM involvement in acute myelogenous leukemia, where a mutated protein lacking a folded C-terminal domain (NPM1c+) has been found in the cytoplasm in patients. This aberrant localization has been linked to the development of the disease, and is associated with improved clinical outcomes. Strategies against this subtype of acute myelogenous leukemia include the refolding of the C-terminal domain using pharmalogical chaperones and the displacement of the protein from nucleolus to nucleoplasm, which has been linked to apoptotic mechanisms. It has also been shown that in the context of clonal hematopoiesis of undetermined significance harboring a DNMT3A mutation, subsequent NPM1 mutations drive progression into overt myeloproliferative neoplasm.

Application

Application	Dilution Ratio
IHC	1:200 - 1:500

Overview

Antibody Type	Recombinant Rabbit monoclonal Antibody
Immunogen	Synthetic peptide within Human Nucleophosmin aa 1-100 (N terminal).
Species Reactivity	Human
Applications	IHC
Predicted Molecular Weight	33 kDa
Positive Control	HeLa cell lysate, HepG2 cell lysate, A431 cell lysate, C2C12 cell lysate, RAW264.7 cell lysate, PC-12 cell lysates, HeLa, C2C12, PC-12, human colon carcinoma tissue, human skin tissue, mouse small intestine tissue, mouse colon tissue, rat spleen tissue, rat kidney tissue, Daudi, HL-60.
Conjugate	Unconjugated
Form	Liquid
Storage Buffer	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype	IgG
Purification Method	Protein A affinity purified.
Uniprot ID	P06748 (Human), Q61937 (Mouse), P13084 (Rat)
Alternative Names	B23, MGC104254, NO38, NPM, NPM_HUMAN, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin (nucleolar phosphoprotein B23 numatrin), Nucleophosmin, nucleophosmin nucleoplasmin family member 1, Nucleophosmin/nucleoplasmin family member 1, Numatrin, OTTHUMP00000161024, OTTHUMP00000161025, OTTHUMP00000223397, OTTHUMP00000223398

Data

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with NPM1/Nucleophosmin Rabbit Monoclonal Antibody(ARA648) at 1/200 dilution.