NOX4 Rabbit Polyclonal Antibody

Datasheet

MSDS

Key features and details

Rabbit polyclonal antibody to NOX4

Target: NOX4
Source/Host: Rabbit
Reactivity: Human,Mouse,Rat,Bovine
Clonality: Polyclonal
Applications: WB
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : APA08089

US$ Please choose

Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

NADPH oxidase that catalyzes predominantly the reduction of oxygen to H2O2 . Can also catalyze to a smaller extent, the reduction of oxygen to superoxide . May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity . May regulate insulin signaling cascade . May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB . May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation . Promotes ferroptosis, reactive oxygen species production and reduced glutathione (GSH) levels by activating NLRP3 inflammasome activation and cytokine release .

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit polyclonal antibody to NOX4
Specificity	Recognizes endogenous levels of NOX4 protein.
Antibody Type	Primary antibody
Imnunogen	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NOX4. The exact sequence is proprietary.
Purification	The antibody was purified by immunogen affinity chromatography.
Molecular Weight	Predicted: 66 kD; Observed: 67 kD
Form/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Names	RENOX; NADPH oxidase 4; Kidney oxidase-1; KOX-1; Kidney superoxide-producing NADPH oxidase; Renal NAD(P)H-oxidase
Gene Symbol	NOX4
Entrez Gene	50507(Human); 50490(Mouse); 85431(Rat)
SwissProt	Q9NPH5(Human); Q9JHI8(Mouse); Q924V1(Rat)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of NOX4 expression in A375 (A), U2OS (B), MCF7 (C), mouse heart (D), rat heart (E) whole cell lysates. (Predicted band size: 66 kD; Observed band size: 67 kD)

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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