Myosin Heavy Chain Rabbit Monoclonal Antibody(ARA910)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
-
Brand:
CAT.NO. : ARA6745
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Size:
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Product Details
Background
Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of myosin have been cloned: smooth muscle myosins, striated muscle myosins and non-muscle myosins. Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of myosin light chain by myosin light chain kinase. Myosin heavy chains are encoded by the MYH gene family and have Actin-activated ATPase activity which generates the motor function of myosin. Myosin heavy chains, which were initially isolated from a human fetal skeletal muscle, are the major determinant in the speed of contraction of skeletal muscle. Various isoforms of myosin heavy chain are differentially expressed depending on the functional activity of the muscle.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
IHC | 1:100-1:500 |
Overview
Antibody Type | Recombinant Rabbit monoclonal Antibody |
Immunogen | Recombinant protein within Human Myosin heavy chain aa 1220-1420 / 1939 |
Molecular Weight | Predicted band size: 224 kDa |
Positive Control | Rat skeletal muscle tissue lysates, human skeletal muscle tissue lysates, human skeletal muscle tissue, mouse skeletal muscle tissue, mouse heart tissue, rat skeletal muscle tissue, rat heart tissue, mouse smooth muscle tissue, human striated muscle tissue, human heart tissue |
Conjugation | unconjugated |
Form/Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide |
Isotype | IgG |
Purification | Protein A affinity purified |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Immunohistochemical staining of human skeletal muscle tissue using Myosin Heavy Chain Rabbit Monoclonal Antibody(ARA910)
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?
AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.
What development scenarios are pathology raw antibodies mainly used for?
Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.
How can I evaluate whether a raw antibody is suitable for our staining platform?
It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.
Can you provide samples for platform validation?
Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.
How should raw antibodies be paired with detection systems?
AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.
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