Myosin 7 Rabbit Polyclonal Antibody
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Gene Symbol:
- Storage:
-
Brand:
CAT.NO. : APA14687
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Product Details
Background
Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing early-onset distal myopathy.
Aplication
|
Application |
Dilution Ratio |
|
WB |
1:500 - 1:1000 |
|
IF/ICC |
1:50 - 1:200 |
Overview
|
Product Description |
Rabbit polyclonal antibody to Myosin 7 |
|
Immunogen |
Recombinant full length protein of human Myosin 7 |
|
Purification Method |
The antibody was purified by immunogen affinity chromatography. |
|
Clonality |
Polyclonal |
|
Form |
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
|
Gene Symbol |
MYH7 |
|
Alternative Names |
MYHCB; Myosin-7; Myosin heavy chain 7; Myosin heavy chain slow isoform; MyHC-slow; Myosin heavy chain, cardiac muscle beta isoform; MyHC-beta |
|
Gene ID (Human) |
4625 |
|
Gene ID (Mouse) |
140781 |
|
Gene ID (Rat) |
29557 |
|
Protein ID (Human) |
P12883 |
|
Protein ID (Mouse) |
Q91Z83 |
|
Protein ID (Rat) |
P02564 |
Data
Western blot analysis of Myosin 7 expression in mouse skeletal muscle (A), rat skeletal muscle (B) whole cell lysates. (Predicted band size: 223 kD; Observed band size: 250 kD)
Immunofluorescent analysis of Myosin 7 staining in rat skeletal muscle. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX®Flour 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for use in diagnostic procedures.
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