Mouse IL-21 ARExSet® antibody pair for ELISA

Protocol Booklet

Mouse IL-21 ARExSet® antibody pair for ELISA

Key features and details

Species: Mouse
Size: 5 plates,15 plates,50 plates
Sample Volume: 10 μl
Standard Curve Range: 62.50 pg/ml-4000 pg/ml
Assay type: Not Ready-to-Use
Sensitivity: 3.76pg/ml(10 μl)
Operation Duration: 120min
Assay type: Sandwich Elisa

Brand:

CAT.NO. : ASEM0020

US$ Please choose

Size:

5 plates 15 plates 50 plates

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

IL-21 (Interleukin-21) is member of the family of cytokines that utilize the common gamma chain as a receptor subunit. This subunit is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15. IL-21 is produced by activated T follicular helper cells (Tfh), Th17 cells, and NKT cells. It exerts its biological effects through a heterodimeric receptor complex of the common gamma chain and the IL-21-specific IL-21 R. IL-21 supports Tfh cell development, immunoglobulin affinity maturation, plasma cell differentiation, B cell memory responses, and the migration of dendritic cells to draining lymph nodes. It costimulates the activation, proliferation, and survival of CD8+ T cells and NKT cells and promotes Th17 cell polarization. It blocks the generation of regulatory T cells and their suppressive effects on CD4+ T cells. In addition, IL-21 suppresses cutaneous hypersensitivity reactions by limiting allergen-specific IgE production and mast cell degranulation.

typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0522	0.0593	0.0558
62.50	0.1525	0.1672	0.1599	0.1041
125.00	0.2517	0.2496	0.2507	0.1949
250.00	0.3852	0.3909	0.3881	0.3323
500.00	0.6523	0.6655	0.6589	0.6032
1000.00	1.1750	1.1000	1.1375	1.0818
2000.00	1.9230	1.9440	1.9335	1.8778
4000.00	3.4543	3.4290	3.4417	3.3859

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

ARExSet® Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.

96 well microplates: AREX Biosciences, Catalog # DSEP01. Plate Sealers: AREX Biosciences, Catalog # DSSF01.

Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2-7.4, 0.2 μm filtered . AREX Biosciences, Catalog # DSCB01.

Blocking Buffer: AREX Biosciences, Catalog # DSBB01.

Wash Buffer: 0.05% Tween 20 in PBS, pH 7.2-7.4. AREX Biosciences, Catalog # DSWB01.

Assay Buffer: 0.5%BSA，0.05%Tween20，PBS Solution.AREX Biosciences, Catalog # DSAB01

Substrate Solution: Tetramethylbenzidine. AREX Biosciences, Catalog # DSTS01.

Stop Solution: 0.5mol/ml H2SO4. AREX Biosciences, Catalog # DSSS01.

New Products

CD74 Mouse Monoclonal Antibody(ARA635)

Human CD74 Mouse Monoclonal Antibody Pair(ARA634/ARA635)

CRBN Rabbit Monoclonal Antibody(ARA644)

HEK293 HCP ELISA Kit

E.coli HCP ELISA Kit