MCM3 Mouse Monoclonal Antibody(C2835)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IHC | 1:100 - 1:500 |
IF/ICC | 1:50 - 1:100 |
FC | 1:100 - 1:200 |
Description | Mouse monoclonal to MCM3 |
Specificity | Recognizes endogenous levels of MCM3 protein |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human MCM3 expressed in E. Coli |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 91 kD; Observed: 105 kD kD |
Form/Buffer | Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | DNA replIC,ation lIC,ensing factor MCM3; DNA polymerase alpha holoenzyme-associated protein P1; P1-MCM3; RLF subunit beta; p102 |
Gene Symbol | MCM3 |
Entrez Gene | 4172(Human) |
SwissProt | P25205(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of MCM3 expression in PC12 (A), HepG2 (B), Hela (C), COS7 (D) whole cell lysates. (Predicted band size: 91 kD; Observed band size: 105 kD kD)
Immunohistochemical analysis of MCM3 staining in human stomach cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of MCM3 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of HeLa cells using Anti-MCM3 Antibody (green) and negative control (red).
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