KRAS Rabbit Monoclonal Antibody(C4065)
Key features and details
- Reactivity:
- Application:
- Host:
- Clonality:
- Target:
-
Brand:
CAT.NO. : AMA03885
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Product Details
Background
K-RAS (GTPase KRas) belongs to the Ras oncogene family. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. It plays an important role in the regulation of cell proliferation and has a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes in colorectal cancer cells in a ZNF304-dependent manner. Mutations in the gene can result in leukemia acute myelogenous, leukemia juvenile myelomonocytic, Noonan Syndrome, gastric cancer, and cardiofaciocutaneous syndrome 2.
Application
|
Application |
Dilution Ratio |
|
WB |
1:500-1:1000 |
|
IHC |
1:50-1:200 |
|
IF/ICC |
1:50-1:200 |
Overview
|
Product Description |
Recombinant rabbit monoclonal antibody to KRAS |
|
Immunogen |
KLH-conjugated synthetic peptide encompassing a sequence within human KRAS protein. The exact sequence is proprietary. |
|
Purification Method |
The antibody was purified by immunogen affinity chromatography. |
|
Clonality |
Monoclonal |
|
Form |
Liquid in PBS, pH 7.3, 50% glycerol, 0.05% BSA, and 0.05% Proclin300. |
|
Gene Symbol |
KRAS |
|
Alternative Names |
KRAS2; RASK2; GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras |
|
Gene ID (Human) |
3845 |
|
Gene ID (Mouse) |
16653 |
|
Gene ID (Rat) |
24525 |
|
Protein ID (Human) |
P01116 |
|
Protein ID (Mouse) |
P32883 |
|
Protein ID (Rat) |
P08644 |
Data
Western blot analysis of KRAS expression in Hela (A) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 22 kD)
Immunohistochemical analysis of KRAS staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of KRAS staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AREX®Flour 488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX®Flour 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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