KRAS Rabbit Monoclonal Antibody(ARA666)
Key features and details
- Reactivity:
- Application:
- Host:
- Clonality:
- Target:
-
Brand:
CAT.NO. : ARA6384
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Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
K-RAS (GTPase KRas) belongs to the Ras oncogene family. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. It plays an important role in the regulation of cell proliferation and has a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes in colorectal cancer cells in a ZNF304-dependent manner. Mutations in the gene can result in leukemia acute myelogenous, leukemia juvenile myelomonocytic, Noonan Syndrome, gastric cancer, and cardiofaciocutaneous syndrome 2.
Application
|
Application |
Dilution Ratio |
|
WB |
1:500-1:1000 |
|
IHC |
1:50-1:200 |
Overview
|
Product Description |
Recombinant rabbit monoclonal antibody to KRAS |
|
Immunogen |
KLH-conjugated synthetic peptide encompassing a sequence within human KRAS protein. The exact sequence is proprietary. |
|
Purification Method |
The antibody was purified by immunogen affinity chromatography. |
|
Clonality |
Monoclonal |
|
Form |
Liquid in PBS, pH 7.3, 50% glycerol, 0.05% BSA, and 0.05% Proclin300. |
|
Gene Symbol |
KRAS |
|
Alternative Names |
KRAS2; RASK2; GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras |
|
Gene ID (Human) |
3845 |
|
Protein ID (Human) |
P01116 |
Data
Western blot analysis of KRAS expression in Hela (A) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 22 kD)
Immunohistochemical analysis of KRAS staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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